AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS

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An Electrophoretic Study of Immune Sera and Purified Antibody Preparations

1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbe...

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AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS*t

Studies on the eleetrophoresis of serum have shown that besides the albumin normal sera possess three separate globulin components differing in mobility and designated as cz, /3, and ~' (1). The relationship of antibodies to these components is of considerable importance. Differences in the antibody globulin formed in various animal species have already been found by ultracentrifugal studies (2...

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Electrophoresis of Purified Antibody Preparations

In connection with a study of the ultracentrifugal sedimentation of antibody preparations (1) the opportunity arose for study of the electrophoretic properties of some of the highly active material under investigation. Measurements of this kind are of value in the characterization of proteins and similar high molecular substances, and may also give information regarding the chemical homogeneity...

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Electrophoresis of Purified Antibody Preparations

In connection with a study of the ultracentrifugal sedimentation of antibody preparations (1) the opportunity arose for study of the electrophoretic properties of some of the highly active material under investigation. Measurements of this kind are of value in the characterization of proteins and similar high molecular substances, and may also give information regarding the chemical homogeneity...

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Electrophoresis of Purified Antibody Preparations

Electrophoretic mobilities of antibody preparations isolated from type specific antipneumococcus horse and rabbit sera, measured over a range of pH values, show that these preparations are distinctly different from normal serum proteins in their electrochemical properties.

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1939

ISSN: 1540-9538,0022-1007

DOI: 10.1084/jem.69.1.119